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the serpine1 overexpression plasmid  (Sino Biological)


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    Structured Review

    Sino Biological the serpine1 overexpression plasmid
    The Serpine1 Overexpression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+serpine1+overexpression+plasmid/sfrp1+overexpression+plasmid/pm36849986-54-3-9
    Average 90 stars, based on 1 article reviews
    the serpine1 overexpression plasmid - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Over Expression:

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCTGACTTCACGAGTCTTT-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer's instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1.
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCT GAC TTC ACG AGT CTT T-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer’s instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Plasmid Preparation:

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCTGACTTCACGAGTCTTT-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer's instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1.
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCT GAC TTC ACG AGT CTT T-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer’s instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Transfection:

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCTGACTTCACGAGTCTTT-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer's instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1.
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCT GAC TTC ACG AGT CTT T-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer’s instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCTGACTTCACGAGTCTTT-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer's instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..

    Article Title: Myricetin alleviated hydrogen peroxide-induced cellular senescence of nucleus pulposus cell through regulating SERPINE1.
    Article Snippet: When the cells reached 80% confluence, the small interfering RNA (siRNA) oligonucleotides targeting SERPINE1 (5′-GCT GAC TTC ACG AGT CTT T-3′) were synthesized by RiboBio (Guangzhou, China). .. In addition, the SERPINE1 overexpression plasmid was obtained from Sino Biological (Beijing, China), and then, Lipofectamine 3000 (Invitrogen, USA) was used to perform the transfection of the plasmids according to the manufacturer’s instructions, and the transfection efficiency of the cells was verified by qPCR after 48 h of transfection at 37 °C. ..



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    Image Search Results


    Primer sequences

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: PAI-1 is a novel component of the miR-17~92 signaling that regulates pulmonary artery smooth muscle cell phenotypes

    doi: 10.1152/ajplung.00137.2017

    Figure Lengend Snippet: Primer sequences

    Article Snippet: PAI-1 overexpression (PAI-OE) plasmids (PAI1-Bio-His, or Serpine1-bio-His) were purchased from Addgene (Cambridge, MA) and transfected into human PASMCs using Lipofectamine 2000.

    Techniques: Luciferase, Plasmid Preparation, Cloning